Koopa gets another present for Kootie Pie - this time he plans to use his Doom Sub to chase everyone out of Venice, Italy then warp the entire city to Dark Land so Kootie Pie can have her own water park. The Mario Bros. are approached in Desert Land by an Italian gondolier and since Mario has a passion for the country of Italy, they couldn't resist. Armed with Frog Suits, can they stop Koopa in time?